Methods The human serum albumin was removed by Hitrap Blue column,and serum was dealed with U9.Then gel was stained by the improved method(0.1% carbinol,0.5% TCA,0.1%Coomassie briliant blue G250).

• 方法用Hitrap Blue层析柱去除血清中白蛋白；用U9对血清样品进行变性处理；采用改进后的方法(0.；1%25甲醇；