Methods The gene was cloned by improved PCR-based subtractive hybridization from apoptotic prostate cancer cell line DU-145 cells induced by ATRA.

• 方法 应用全反式维甲酸诱导前列腺癌细胞系DU-145细胞凋亡，采用基于PCR的改良消减杂交技术克隆与凋亡相关的基因。