Methods The gene sequence encoding human myoglobin(hMb) from the total RNA of human skeletal muscle by RT-PCR and clone into prokaryotic expression vector pRSETc for expression in E.

• 方法应用RT-PCR方法从人骨骼肌总RNA中扩增肌红蛋白基因(hMb)编码序列；克隆入原核表达载体pRSETc中；并在大肠杆菌E.