Methods The endothelial cells of 91 corneal buttons obtained at the time of penetrating keratoplasty (PKP) were stained by 1%alizarin red and 0.25%trypan blue and observed under biomicroscope.

• 方法对91片穿透性角膜移植术时取下的病变角膜片，用1%25茜素红和0.；25%25锥蓝对角膜内皮细胞联合染色，生物显微镜观察其内皮细胞的形态及细胞核的变化。