Methods The SCSA explores the metachromatic properties of acridine orange (AO) and flow cytometry to monitor the susceptibility of sperm chromatin DNA to acid induced denaturation in situ because of the low pH treatment.

• 方法利用吖啶橙的异染性和流式细胞术检测精子染色质DNA对原位酸诱导变性的敏感性。