Methods The ABCB4 gene exon 23 in 31 women with ICP were amplified using polymerase chain reaction (PCR). The PCR products were further run for DNA sequencing so as to detect point mutation.

• 方法:采用聚合酶链反应(PCR)方法对31例妊娠期肝内胆汁淤积症患者扩增ABCB4基因外显子23,PCR产物进行DNA序列测定,以检测突变情况。