Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS.At the presence of EDPC, T 2HS conjugated with BSA,then BSA T 2HS was prepared.

• 方法T-2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应,生成T-2-半琥珀酸酐后,再与小牛血清白蛋白经碳二亚胺偶联结合生成T-2毒素抗原。