Methods T 2 toxin in pyridine reacted with succinic anhydride in a steam bath and converted to T 2HS. At the presence of EDPC, T 2HS conjugated with BSA,then BSA T 2HS was prepared.

• 方法 T- 2毒素与琥珀酸酐在吡啶参与下于蒸汽浴中反应 ,生成 T- 2 -半琥珀酸酐后 ,再与小牛血清白蛋白经碳二亚胺偶联结合生成 T- 2毒素抗原。