Methods Recombinant GDH was used to immunize Balb/C mice and the mAbs against GDH were prepared using hybridoma technique followed by identification of IgG isotype and its affinity.

• 方法用基因重组的GDH蛋白免疫Balb/C小鼠,采用杂交瘤细胞技术制备McAb,测定其免疫球蛋白亚类及其亲和力。