Methods Rat BM-MNCs were isolated by centrifugation through a Histopaque density gradient and cultured for four weeks in itro.

• 方法从大鼠胫骨及股骨采集骨髓,密度梯度离心法分离出骨髓单个核细胞进行体外培养,并连续收集4周培养上清液。