Methods Rabbit cardiac myocytes were isolated with low calcium, protease and collagenase successively and the myocytes were cultured with suspending and adherent method.

• 方法依次用低钙、蛋白酶及胶原酶灌流兔心脏,分离心肌细胞,并用悬浮法和贴壁法进行无血清原代培养。