Methods RNA was isolated from the human osteosarcoma cell line U-20S by single-step method of acid guanidinium isothiocyanate phenol-chloroform extraction.

• 方法 采用异硫氰酸胍一步法从人骨肉瘤细胞株U-2OS 中提取总RNA，以逆转录一聚合酶链反应（Reverse Transcription coupled Polymerase Chain Reaction，RT-PCR）扩增cDNA基因片段。