Methods Mutation analysis of the Cx32, MPZ and PMP22 genes were performed by PCR-RFLP, PCR-SSCP , PCR-DGGE and/or direct sequencing in 32 CMT probands of the Hans in China.

• 方法 分别应用PCR 双酶切、聚合酶链反应 单链构象多态性分析 (PCR SSCP)、聚合酶链反应 变性梯度凝胶电泳 (PCR DGGE)或PCR直接测序等方法对 32个确诊的CMT家系进行了PMP2 2、MPZ、Cx32等致病基因的突变检测。