Methods Mtb-Ag and its two protein components were used to stimulate T cell sub-populations of normal subjects in different doses and the responded cells were phenotyped by flow cytometry.

• 方法 用快速蛋白液相色谱仪（FPLC）定性分析Mtb-Ag的蛋白组成,并用不同剂量的Mtb-Ag及其纯化组分体外刺激健康人外周血PBMC,培养9d后经流式细胞仪检测细胞表型。