Methods Mononuclear cells were isolated from human peripheral blood cells by density gradient centrifugation and cultured in M199 medium with fetal blood serum(FBS),vascular endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF).

• 方法用密度梯度离心法从人外周血中分离单个核细胞。 在加有胎牛血清(fetal blood serum,FBS)、血管内皮生长因子(vascular end othelial growthfactor,VEGF)和碱性成纤维生长因子(basic fibroblast growthfactor,bFGF)的M199培养液中培养。