Methods Mononuclear cells (MNC) were separated from cord blood with both 6% hetastarch (HES) and Ficoll-Paque. Then CD133+ cells were purified with MACS (magnetic activated cell-sorting system) CD133 cell isolation kit.

• 方法6%25羟乙基淀粉(HES)和Ficoll-Paque联合应用分离出脐血中单个核细胞,再用MACSCD133磁珠分离试剂盒分选其中的CD133+细胞。