Methods Model of vasoligation and vasostomy in male rat was established, and then ter- minal deoxynucleotidyl transferase-mediated dUTP nick labelling technique to detect the apoptosis of spermato- genic cells at 4, 8, 12, 16 weeks after vasostomy.

• 方法 以雄性 SD大鼠为研究对象 ,建立输精管阻断及阻断后复通模型 ,采用 DNA缺口原位标记凋亡细胞方法 ,观察大鼠生精细胞在输精管复通后 4、8、12及 16周的凋亡状况。