Methods Lipofectin method was used to transfect pCR3.1/CCK_2R vector expressing gastrin receptor into a colon cancer cell line col0320.Gastrin and gastrin antagonist were used to up-regulate and down-regulate the signaling pathway,respectively.

• 方法采用脂质体介导法；将胃泌素受体(CCK_2R)的真核表达载体pCR3.；1/CCK_2R稳定转染人结肠癌细胞株colo320；上调胃泌素的作用；