Methods L 16 (4 5) orthogonal test was applied to analyse the four factors, including culture medium, ways of heat shock, dilution degrees of AJC and pH value. The results were comprehensively scored according to the number of colonies.

• 方法 采用L16 (45)正交试验,对培养基种类、pH值、热休克方式、果汁稀释度四个影响浓缩苹果汁中嗜酸耐热菌检测的因素进行了研究,以菌落数多少进行综合评分,优选最佳检测方法。