Methods Immunize New Zealand rabbits,sheep and dogs with purified rOspA at different dosages. Determine the serum specific IgG by IFA and perform neutralization test in vitro.

• 方法采用纯化的rOspA作抗原,以新西兰家兔、绵羊和犬为试验动物,分不同剂量组和对照组进行免疫,用间接免疫荧光法(IFA)检测其血清特异性抗体(IgG),并进行体外中和试验。