Methods IDO activity is indirectly determined by detecting the amount of tryptophan and kynurenine with FLD and DAD respectively. The ability to restrict growth of T. gondii was evaluted by 3H-U incroporation assay.

• 方法 以荧光检测器和紫外检测器分别检测色氨酸和犬尿氨酸 ,间接测定IDO活性 ,采用3H -尿嘧啶 (3H -U)特异标记的弓形虫在HMF内的增殖实验。