Methods Horseradish peroxidase(HRP) retrograde tracing combined with biotinylated dextran amine (BDA) anterograde tracing technique was used. The labeling results were observed under light of electron microscope,respectively.

• 方法 HRP逆行追踪与生物素葡聚糖胺 (BDA)顺行追踪相结合的双标技术 ,标记结果分别在光镜或电镜下观察。