Methods Genomic DNA was isolated, and cytochrome c oxidase I (COI) and cytochrome b (Cyt b ) genes were amplified by polymerase chain reaction (PCR).

• 方法 实验室饲养的F1钉螺 ；单个抽提基因组DNA ；PCR扩增细胞色素c氧化酶I(COI)、细胞色素b(Cytb)基因并测序 ；用GENETYX MACver .