Methods Full length HMGB1 cDNA cloned by PCR from mammary library was inserted into pET28a(+) vectors to construct pET28a(+)-HMGB1 plasmid. After confirmation, the positive pET28a(+)-HMGB1 recombinant plasmid was transformed into E.

• 方法应用PCR从乳腺文库中扩增得到HMGB1基因片段,并将其克隆到pET28a(+)载体上,对获得的pET28a(+)-HMGB1重组质粒扩增后进行PCR、酶切和测序鉴定。