Methods Eosinophil cationic protein(ECP) mRNA levels in peripheral blood were measured in 40 patients with asthma and 20 normal controls by semi-quantitative analysis with reverse transcriptional-polymerase chain reaction(RT-PCR).

• 方法采用逆转录聚合酶链反应技术(RT-PCR),对40例哮喘患者及20例正常对照者的ECP mRNA表达水平进行半定量分析。