Methods Effects of xy9902 on the proliferation and differentiation of MC3T3-E1 cells was determined by MTT and ALP activity measurement.

• 方法采用MTT比色法测定化合物对成骨细胞MC3T3-E1的增殖作用; 通过硝基苯磷酸盐法测定细胞内碱性磷酸酶(A lkaline phosphatase,ALP)活性的变化,观察化合物对细胞分化的影响;