Methods Dorsal root ganglions (DRG) from rat embryos were isolated under microscope and dissociated in trypsin.The dissociated neurons were treated with 5-fluorouracil for purification.
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方法取胚胎大鼠背根神经节,通过胰蛋白酶消化、抗有丝分裂药物纯化等方法获得背根神经节神经元。
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