Methods DNA was extracted from peripheral blood of the proband and her parents. The GJB2,GJB6 and GJB3 gene mutations were analyzed by direct sequencing PCR products.
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释义
方法采集先证者及其父母外周血并提取DNA,对GJB2、GJB6、GJB3基因编码区进行PCR扩增,以直接测序的方法进行突变分析。
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