Methods Clone CN54 Nef gene into vector pThioHisA and transform the constructed recombinant plasmid to E. coli BL21 Codonplus-RIL for expression under induction of IPTG.

• 方法将CN54 Nef基因克隆至pThio-HisA载体,构建非融合表达载体,转化大肠杆菌BL21 Codonplus-RIL,IPTG诱导表达,通过尿素梯度洗涤包涵体纯化Nef蛋白,透析复性。