Methods CD34 + stem cells were obtained from cord blood by MACS method, CD3, CD16 and CD56 molecules expressed on cell surface were detected by Flow Cytometer. MTT method was used to detect the cytotoxicity of NK cells.

• 方法 采用MACS法分离脐血中CD34 +细胞 ,分别用IL 15、SCF(造血干细胞因子 )和IL 15 +SCF体外培养 ,流式细胞仪测定CD3、CD16、CD5 6分子 ,MTT法检测NK细胞杀伤活性。