Methods By using Northern blot and Western blot methods,the expression of gene and protein of VEGF-A,-C in all 6 tested cell lines (ASPC-1,CAPAN-1,MIA-PaCa-2,PANC-1,COLO-357 and T3M4) was detected respectively.

• 方法 用Northern杂交和Western杂交法分别分析人胰腺癌细胞株(ASPC 1、CAPAN 1、MIA PaCa 2、PANC 1、COLO 3 5 7和T3M 4)中VEGF A、C基因和蛋白的表达;