Methods Both vessels occlusion and immunofluorescent methods in gerbil hippocampal tissue slices incubated in vitro were used to observe change of GFAP concentration and distribution.

• 方法钳夹沙土鼠的双侧颈总动脉制造脑缺血模型,体外孵育海马组织脑片,应用免疫荧光法染色,观察GFAP浓度的变化及分布。