Methods BALB/c mice were passively sensitized by intravenous infection of anti-DNP IgE monoclonal antibody 24 h before DNFB challenge. Skin reaction was elicited by applying DNFB to both sides of each ear of sensitized mice.

• 方法采用BALB/c 小鼠尾静脉注射 anti-DNP IgE 单克隆抗体,以2,4-二硝基氟苯(DNFB)激发,建立皮肤三相过敏模型。