Methods All patient samples, first marked with CD42-PE monoclonal antibody, are stained with the fluorescent KNA-dye Thiazole Orange(TO) ,then RP Counts refered to normal subjects are obtained by flow cytometry.

• 方法 运用双标记法,用血小板特异性抗体CD42-PE标记血小板,再用噻唑橙(Thiazole Orange,TO)染色rp内RNA,以健康人为对照,用流式细胞仪检测血液病患者在初诊、初治、缓解期的网织血小板水平。