Methods After treatment of amino acids with 2,4 dinitrofluorobenzene(DNFB) excessive DNFB was removed by extraction with tert bultyl methyl ether.

• 方法 氨基酸与2 ,4 二硝基氟苯衍生化反应完成后 ,用 10ml叔丁基甲醚抽提 2次 ,每次 5ml,以除去多余衍生化试剂。