Methods After the rat glioma model was established,BK was infused through intracarotid artery,the distribution and expression of Kir6.2 protein were determined by immunohistochemistry and western blot analysis.
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方法建立大鼠脑胶质瘤模型;颈内动脉灌注缓激肽后;采用免疫组化SABC法和Western blot法测定肿瘤组织ATP敏感性钾通道蛋白的功能亚基Kir6.;2的分布和表达的变化。
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