Methods Acutely isolated myocytes from adult rat and cultured neonatal cardiomyocytes were immunostained with antibodies against to actin and tropon, respectively.

• 方法采用急性分离的成年大鼠心肌细胞和培养的乳鼠心肌细胞为标本,分别用免疫荧光染色直接法和间接法对细胞的肌动蛋白和肌钙蛋白进行染色。