Methods A set of universal primers was designed based on the conservative regions in bacterial 16S ribosomal RNA genes. 7 control bacterial strains,human DNA,HBV-DNA and 37 samples of ascites of patients with cirrhosis were amplified with PCR.

• 方法在细菌16S rRNA基因保守区设计一对通用引物,对7种对照菌株、人基因组DNA、HBV-DNA、37份肝硬化患者腹水进行PCR扩增。