Methods A rat model of cerebral ischemia was established. The expression of Ngb mRNA in different time after cerebral ischemia was detected by one step RT-PCR method and computer image analysis system.

• 方法建立Wistar大鼠脑组织缺血模型,应用一步法逆转录-聚合酶链式反应(one step RT-PCR)技术与计算机图像分析技术检测脑组织缺血后不同时间Ngb mRNA表达的差别。