Methods A pair of oligonucleotide primers were designed with hylA gene as target sequence. Fiftyfour strain of standard LM and 21 strains of irrelevant bacteria were amplified and confirmed by PCR technique.

• 方法选取hlyA基因作为靶序列设计一对引物，用该引物对54株标准李斯特菌和21株无关菌进行pcr扩增，得到进一步验证。