Methods A DDP-resistant human ovarian adenocar- cinoma cell subline (COC1 /DDP ) was developed by contineous stepwise selection in increasing concentra- tion of DDP from the parent cell line COC1 in vitro. The multiple changes of biological.

• 方法应用顺铂连续作用并逐步提高药量的递增压力选择法，从人卵巢腺癌细胞COC1中分离出对顺铂耐药的细胞亚株（COC1/DDP），并比较耐药细胞和亲代细胞某些生物学特性差异。