Method:Used AFP which purified from human cord serum,act on human hepatoma Bel7402cell which cultured in vitro. Utilized the MTT counts,cell cycle phase analysis with flow cytomtry and 3 H-thymidine deoxyribonucleotide( 3 H-TdR)incorporation.

• 方法:用从人脐带血清中提取的甲胎蛋白,作用于体外培养的人肝癌Bel7402细胞,运用MTT染色计数法,流式细胞仪测定细胞周期和3H-脱氧胸腺嘧啶核苷(3H-TdR)参入法等指标检测细胞增殖状况; 放射受体分析细胞膜上甲胎蛋白受体存在和数目;