Method: Immunohistochemical techniques are employed to detect expression ofplk1 raf-1 and Ki-67 in 80 cases of human gastric cancer and 15 cases ofnormal gastric tissues.

• 方法：采用免疫组织化学SP法检测80例胃癌组织和15例癌旁正常胃组织中plk1、raf-1和Ki-67的表达水平。