Method Rat islets were isolated with in situ ductal collagenase distention, stationary digestion and dextran gradient separation.

• 方法本实验经胰管注射胶原酶、胰腺静止消化分离成年大鼠胰岛,葡聚糖离心纯化,获得胰岛细胞,进行记数和功能鉴定。