Method In vitro: primary hippocampus neurons were cultured using embryo rat brains, the MTT and the activity of LDH were assayed and the pathology was observed, so as to study the protection of Tet from excitotoxicity induced by quinolinic acid(QA).

• 方法：离体实验：采用海马神经元原代培养，检测培养神经元的MTT、 LDH活性，并观察其病理形态学改变，来研究Tet对QA损伤的海马神经元的保护作用。