Method First,the rat model of depression due to chronic stress was chronically given CIF,and the cortices of the model rats were then separated for detecting the B_ max and K_d of 5-HT_1 and 5-HT_2 receptors by radioligand binding assay.

• 方法 应用大鼠慢性应激模型慢性给药 ,取动物大脑皮层组织 ,采用放射配基结合法测定皮层 5 HT1受体和 5 HT2 受体的最大结合率 (Bmax)和平衡解离常数(Kd)。