Method Aft er isolation and culture, Legionella colonies were preliminarily distinguished o n blood plate(negative) and plate without L-cysteine. The bacteria was confirme d by serotyping and PCR.

• 方法用分离培养法,依据菌落形态和在缺乏L-半胱氨酸的平板和血平板不生长初判军团菌,再用血清凝集进行分型,结合生化反应和pcr结果进行确证。