Method :DNA of Rickecttsia tsutsugamushi was detected by using NPCR and the specific isolation products from the DNA were used for DNA cloning and the DNA sequence analysis.

• 结果:序列分析表明扩增的片段为立克次体DNA片段。39例不明原因发热患者血液用NPCR法检出13例恙虫病立克次体DNA。