Matrix CHCA was prepared in ACN: methanol: water (1:1:1) with 0.1% formic acid and 0.01 M 18-crown-6.This protocol could be used to differentiate B. anthracis, S. aureus, E. coli, Y.

• 基质溶液为CHCA溶于乙睛:甲醇:水(l:l:l)，含有0.；1%25甲酸和0