Marrow mesenchymal stem cells can obtain both with adherence separation and density gradient centrifugation by suitable culturing condition. Import FBS and F12-DMEM nutritive medium are better choice.

• 采用全骨髓贴壁分离法和密度梯度离心法,只要选择合适的培养条件均可获得骨髓间充质干细胞,但选用进口胎牛血清、F12-DMEM培养基效果较好。